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Photophysics and optical switching in green fluorescent protein mutants

We demonstrate by using low-temperature high-resolution spectroscopy that red-shifted mutants of green fluorescent protein are photo-interconverted among three conformations and are, therefore, not photostable “one-color” systems as previously believed. From our experiments we have further derived t...

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Main Authors: Creemers, T. M. H., Lock, A. J., Subramaniam, V., Jovin, T. M., Völker, S.
Formato: Artigo
Idioma:Inglês
Publicado: The National Academy of Sciences 2000
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC16176/
https://ncbi.nlm.nih.gov/pubmed/10716703
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