Spatially Resolved Fluorescence Correlation Spectroscopy Using a Spinning Disk Confocal Microscope

We develop an extension of fluorescence correlation spectroscopy (FCS) using a spinning disk confocal microscope. This approach can spatially map diffusion coefficients or flow velocities at up to ∼10(5) independent locations simultaneously. Commercially available cameras with frame rates of 1000 Hz...

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Detalhes bibliográficos
Main Authors: Sisan, Daniel R., Arevalo, Richard, Graves, Catherine, McAllister, Ryan, Urbach, Jeffrey S.
Formato: Artigo
Idioma:Inglês
Publicado em: Biophysical Society 2006
Assuntos:
Spectroscopy, Imaging, Other Techniques
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1635679/
https://ncbi.nlm.nih.gov/pubmed/16950838
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.106.084251
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