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Site-specific incorporation of 3-nitrotyrosine as a probe of pK(a) perturbation of redox-active tyrosines in ribonucleotide reductase

E. coli ribonucleotide reductase catalyzes the reduction of nucleoside 5′-diphosphates into 2′-deoxynucleotides and is composed of two subunits: α2 and β2. During turnover, a stable tyrosyl radical (Y·) at Y(122)-β2 reversibly oxidizes C(439) in the active site of α2. This radical propagation step i...

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Библиографические подробности
Главные авторы: Yokoyama, Kenichi, Uhlin, Ulla, Stubbe, JoAnne
Формат: Artigo
Язык:Inglês
Опубликовано: 2010
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Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC2905227/
https://ncbi.nlm.nih.gov/pubmed/20518462
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja101097p
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