Expression profiling via novel multiplex assay allows rapid assessment of gene regulation in defined signalling pathways

The current interest in expression of groups of functionally related genes creates a demand for novel experimental tools. We describe a multiplex ligation-dependent amplification procedure (RT-MLPA), which accurately quantifies up to 45 transcripts of interest in a one-tube assay. The output, a set...

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Detalhes bibliográficos
Main Authors: Eldering, Eric, Spek, C. Arnold, Aberson, Hella L., Grummels, Annette, Derks, Ingrid A., de Vos, Alex F., McElgunn, Cathal J., Schouten, Jan P.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2003
Assuntos:
NAR Methods Online
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC290288/
https://ncbi.nlm.nih.gov/pubmed/14627843
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gng153
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