Real-time monitoring of nucleic acid ligation in homogenous solutions using molecular beacons

Nucleic acids ligation is a vital process in the repair, replication and recombination of nucleic acids. Traditionally, it is assayed by denatured gel electrophoresis and autoradiography, which are not sensitive, and are complex and discontinuous. Here we report a new approach for ligation monitorin...

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Bibliographic Details
Main Authors: Tang, Zhiwen, Wang, Kemin, Tan, Weihong, Li, Jun, Liu, Lingfeng, Guo, Qiuping, Meng, Xiangxian, Ma, Changbei, Huang, Shasheng
Format: Artigo
Language:Inglês
Published: Oxford University Press 2003
Subjects:
NAR Methods Online
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC290283/
https://ncbi.nlm.nih.gov/pubmed/14627838
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gng146
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