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Purification of Restriction Fragments Containing Replication Intermediates from Complex Genomes

In order to perform 2-D gel analyses on restriction fragments from higher eukaryotic genomes, it is necessary to remove most of the linear, non-replicating, fragments from the starting DNA preparation. This is because the replication intermediates in a single-copy locus constitute such a minute frac...

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Detalhes bibliográficos
Main Authors: Mesner, Larry D., Dijkwel, Pieter A., Hamlin, Joyce L.
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2902164/
https://ncbi.nlm.nih.gov/pubmed/19563104
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