First siRNA library screening in hard-to-transfect HUVEC cells

Meaningful RNAi-based data for target gene identification are strongly dependent on the use of a biologically relevant cell type and efficient delivery of highly functional siRNA reagents into the selected cell type. Here we report the use of the Amaxa® Nucleofector® 96-well Shuttle® System for siRN...

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Detalhes bibliográficos
Principais autores: Zumbansen, Markus, Altrogge, Ludger M, Spottke, Nicole UE, Spicker, Sonja, Offizier, Sheila M, Domzalski, Sandra BS, St Amand, Allison L, Toell, Andrea, Leake, Devin, Mueller-Hartmann, Herbert A
Formato: Artigo
Idioma:Inglês
Publicado em: Library Publishing Media 2009
Assuntos:
Technology Report
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2902142/
https://ncbi.nlm.nih.gov/pubmed/20628494
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