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Engineering of Clostridium phytofermentans Endoglucanase Cel5A for Improved Thermostability
A family 5 glycoside hydrolase from Clostridium phytofermentans was cloned and engineered through a cellulase cell surface display system in Escherichia coli. The presence of cell surface anchoring, a cellulose binding module, or a His tag greatly influenced the activities of wild-type and mutant en...
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| Główni autorzy: | , , , |
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| Format: | Artigo |
| Język: | Inglês |
| Wydane: |
American Society for Microbiology (ASM)
2010
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| Hasła przedmiotowe: | |
| Dostęp online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2901741/ https://ncbi.nlm.nih.gov/pubmed/20511418 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00958-10 |
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