Cloning, Sequencing, and Expression of a Eubacterium cellulosolvens 5 Gene Encoding an Endoglucanase (Cel5A) with Novel Carbohydrate-Binding Modules, and Properties of Cel5A

A novel Eubacterium cellulosolvens 5 gene encoding an endoglucanase (Cel5A) was cloned and expressed in Escherichia coli, and its enzymatic properties were characterized. The cel5A gene consists of a 3,444-bp open reading frame and encodes a 1,148-amino-acid protein with a molecular mass of 127,047...

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Detalhes bibliográficos
Main Authors: Yoda, Kazutoyo, Toyoda, Atsushi, Mukoyama, Yoshihiro, Nakamura, Yutaka, Minato, Hajime
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2005
Assuntos:
Enzymology and Protein Engineering
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1265948/
https://ncbi.nlm.nih.gov/pubmed/16204489
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.10.5787-5793.2005
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