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Engineering of Clostridium phytofermentans Endoglucanase Cel5A for Improved Thermostability

A family 5 glycoside hydrolase from Clostridium phytofermentans was cloned and engineered through a cellulase cell surface display system in Escherichia coli. The presence of cell surface anchoring, a cellulose binding module, or a His tag greatly influenced the activities of wild-type and mutant en...

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Detalhes bibliográficos
Main Authors: Liu, Wenjin, Zhang, Xiao-Zhou, Zhang, Zuoming, Zhang, Y.-H. Percival
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2901741/
https://ncbi.nlm.nih.gov/pubmed/20511418
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00958-10
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