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On Relevance of Codon Usage to Expression of Synthetic and Natural Genes in Escherichia coli

A recent investigation concluded that codon bias did not affect expression of green fluorescent protein (GFP) variants in Escherichia coli, while stability of an mRNA secondary structure near the 5′ end played a dominant role. We demonstrate that combining the two variables using regression trees or...

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Bibliographic Details
Main Authors: Supek, Fran, Šmuc, Tomislav
Format: Artigo
Language:Inglês
Published: Genetics Society of America 2010
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2900969/
https://ncbi.nlm.nih.gov/pubmed/20421604
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/genetics.110.115477
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