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A Continuous Kinetic Assay for Adenylation Enzyme Activity and Inhibition

Adenylation/adenylate-forming enzymes catalyze the activation of a carboxylic acid at the expense of ATP to form an acyl-adenylate intermediate and pyrophosphate (PP(i)). In a second half-reaction, adenylation enzymes catalyze the transfer of the acyl moiety of the acyl-adenylate onto an acceptor mo...

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Detalhes bibliográficos
Main Authors: Wilson, Daniel J., Aldrich, Courtney C.
Formato: Artigo
Idioma:Inglês
Publicado em: 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2900519/
https://ncbi.nlm.nih.gov/pubmed/20450872
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2010.04.033
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