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Secretion of an Endogenous Subtilisin by Pichia pastoris Strains GS115 and KM71
The methylotrophic yeast Pichia pastoris is widely used for the expression of heterologous enzymes. While the purity of the desired expression product is of major importance for many applications, we found that recombinant enzymes produced in methanol medium were contaminated by a 37-kDa endogenous...
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| Hlavní autoři: | , , , , |
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| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
American Society for Microbiology (ASM)
2010
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2897435/ https://ncbi.nlm.nih.gov/pubmed/20472730 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00412-10 |
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