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Secretion of an Endogenous Subtilisin by Pichia pastoris Strains GS115 and KM71

The methylotrophic yeast Pichia pastoris is widely used for the expression of heterologous enzymes. While the purity of the desired expression product is of major importance for many applications, we found that recombinant enzymes produced in methanol medium were contaminated by a 37-kDa endogenous...

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Bibliografski detalji
Glavni autori: Salamin, Karine, Sriranganadane, Dev, Léchenne, Barbara, Jousson, Olivier, Monod, Michel
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology (ASM) 2010
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2897435/
https://ncbi.nlm.nih.gov/pubmed/20472730
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00412-10
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