Glycoprotein production for structure analysis with stable, glycosylation mutant CHO cell lines established by fluorescence-activated cell sorting

Stable mammalian cell lines are excellent tools for the expression of secreted and membrane glycoproteins. However, structural analysis of these molecules is generally hampered by the complexity of N-linked carbohydrate side chains. Cell lines with mutations are available that result in shorter and...

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Autori principali: Wilke, Sonja, Krausze, Joern, Gossen, Manfred, Groebe, Lothar, Jäger, Volker, Gherardi, Ermanno, van den Heuvel, Joop, Büssow, Konrad
Natura: Artigo
Lingua:Inglês
Pubblicazione: Wiley Subscription Services, Inc., A Wiley Company 2010
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2895251/
https://ncbi.nlm.nih.gov/pubmed/20512979
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.390
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