Glycoprotein production for structure analysis with stable, glycosylation mutant CHO cell lines established by fluorescence-activated cell sorting

Stable mammalian cell lines are excellent tools for the expression of secreted and membrane glycoproteins. However, structural analysis of these molecules is generally hampered by the complexity of N-linked carbohydrate side chains. Cell lines with mutations are available that result in shorter and...

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Main Authors: Wilke, Sonja, Krausze, Joern, Gossen, Manfred, Groebe, Lothar, Jäger, Volker, Gherardi, Ermanno, van den Heuvel, Joop, Büssow, Konrad
Format: Artigo
Language:Inglês
Published: Wiley Subscription Services, Inc., A Wiley Company 2010
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2895251/
https://ncbi.nlm.nih.gov/pubmed/20512979
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.390
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