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Transforming a Pair of Orthogonal tRNA-aminoacyl-tRNA Synthetase from Archaea to Function in Mammalian Cells
A previously engineered Methanocaldococcus jannaschii [Image: see text]–tyrosyl-tRNA synthetase pair orthogonal to Escherichia coli was modified to become orthogonal in mammalian cells. The resulting [Image: see text]-tyrosyl-tRNA synthetase pair was able to suppress an amber codon in the green fluo...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2889833/ https://ncbi.nlm.nih.gov/pubmed/20582317 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0011263 |
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