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Semisynthesis of NaK; a Na(+) and K(+) conducting ion channel
In this contribution, we describe the semisynthesis of NaK, a bacterial non-selective cation channel. In the semisynthesis, the NaK polypeptide is assembled from a recombinantly expressed thioester peptide and a chemically synthesized peptide using the native chemical ligation reaction. We describe...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2889250/ https://ncbi.nlm.nih.gov/pubmed/20415433 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi100413z |
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