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Plate capture assay of fluorescent oligonucleotide duplex reporter-transcription factor complexes

We previously developed prototype oligodeoxyribonucleotide (ODN) duplex fluorescence energy transfer (FRET) reporters for optical sensing of NF-κB transcription factor. We report here a plate-binding assay designed for optimizing the above reporters. Nitrilotriacetate-bearing plates were prepared by...

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Bibliografiske detaljer
Main Authors: Zhang, Surong, Bogdanov, Alexei
Format: Artigo
Sprog:Inglês
Udgivet: 2009
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2889241/
https://ncbi.nlm.nih.gov/pubmed/19572630
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bc900137y
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