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Plate capture assay of fluorescent oligonucleotide duplex reporter-transcription factor complexes
We previously developed prototype oligodeoxyribonucleotide (ODN) duplex fluorescence energy transfer (FRET) reporters for optical sensing of NF-κB transcription factor. We report here a plate-binding assay designed for optimizing the above reporters. Nitrilotriacetate-bearing plates were prepared by...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2889241/ https://ncbi.nlm.nih.gov/pubmed/19572630 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bc900137y |
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