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Direct role for the RNA polymerase domain of T7 primase in primer delivery

Gene 4 protein (gp4) encoded by bacteriophage T7 contains a C-terminal helicase and an N-terminal primase domain. After synthesis of tetraribonucleotides, gp4 must transfer them to the polymerase for use as primers to initiate DNA synthesis. In vivo gp4 exists in two molecular weight forms, a 56-kDa...

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Detalhes bibliográficos
Main Authors: Zhu, Bin, Lee, Seung-Joo, Richardson, Charles C.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2889121/
https://ncbi.nlm.nih.gov/pubmed/20439755
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1004220107
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