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Dilute-‘N’-Go dideoxy sequencing of all DNA strands generated in multiplex LATE-PCR assays

We have recently described a Dilute-‘N’-Go protocol that greatly simplifies preparation and sequencing of both strands of an amplicon generated using linear-after-the-exponential (LATE)-PCR, an advanced form of asymmetric PCR . The same protocol can also be used to sequence all limiting primer stran...

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Autors principals: Jia, Yanwei, Osborne, Adam, Rice, John E., Wangh, Lawrence J.
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 2010
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2887968/
https://ncbi.nlm.nih.gov/pubmed/20189962
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkq111
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