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Generating knockout rats by transposon mutagenesis in spermatogonial stem cells

Disrupting genes in the rat on a genome-wide scale will allow the investigation of many biological processes linked to human health. Here we used transposon-mediated mutagenesis to knock out genes in rat spermatogonial stem cells. Given the capacity of the testis to support spermatogenesis from thou...

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Autors principals: Izsvák, Zsuzsanna, Fröhlich, Janine, Grabundzija, Ivana, Shirley, James R, Powell, Heather M, Chapman, Karen M, Ivics, Zoltán, Hamra, F Kent
Format: Artigo
Idioma:Inglês
Publicat: 2010
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2886193/
https://ncbi.nlm.nih.gov/pubmed/20473302
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1461
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