Assignment strategies for large proteins by magic-angle spinning NMR: the 21-kDa disulfide bond forming enzyme DsbA

We present strategies for chemical shift assignments of large proteins by magic-angle spinning (MAS) solid-state NMR (SSNMR), using the 21-kDa disulfide bond forming enzyme DsbA as a prototype. Previous studies have demonstrated that complete de novo assignments are possible for proteins up to ~17 k...

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Hauptverfasser: Sperling, Lindsay J., Berthold, Deborah A., Sasser, Terry L., Jeisy-Scott, Victoria, Rienstra, Chad M.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2010
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2880403/
https://ncbi.nlm.nih.gov/pubmed/20394752
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2010.04.012
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