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Assignment strategies for large proteins by magic-angle spinning NMR: the 21-kDa disulfide bond forming enzyme DsbA
We present strategies for chemical shift assignments of large proteins by magic-angle spinning (MAS) solid-state NMR (SSNMR), using the 21-kDa disulfide bond forming enzyme DsbA as a prototype. Previous studies have demonstrated that complete de novo assignments are possible for proteins up to ~17 k...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2880403/ https://ncbi.nlm.nih.gov/pubmed/20394752 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2010.04.012 |
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