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A device for automated hydrodynamic shearing of genomic DNA

We describe a device for automated fragmentation of genomic DNA by hydrodynamic shearing using a filter screen with uniform pores. Human genomic DNA can be fragmented reproducibly to a range of 2000–12,000 bp by using various fluid flow rates and screens with 0.5–10-µm pores. The utilization of disp...

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Bibliografski detalji
Glavni autori: Joneja, Aric, Huang, Xiaohua
Format: Artigo
Jezik:Inglês
Izdano: 2009
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2880395/
https://ncbi.nlm.nih.gov/pubmed/19594456
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/000113123
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