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A device for automated hydrodynamic shearing of genomic DNA
We describe a device for automated fragmentation of genomic DNA by hydrodynamic shearing using a filter screen with uniform pores. Human genomic DNA can be fragmented reproducibly to a range of 2000–12,000 bp by using various fluid flow rates and screens with 0.5–10-µm pores. The utilization of disp...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2880395/ https://ncbi.nlm.nih.gov/pubmed/19594456 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/000113123 |
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