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Minimizing off-target signals in RNA fluorescent in situ hybridization

Fluorescent in situ hybridization (FISH) techniques are becoming extremely sensitive, to the point where individual RNA or DNA molecules can be detected with small probes. At this level of sensitivity, the elimination of ‘off-target’ hybridization is of crucial importance, but typical probes used fo...

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Detalhes bibliográficos
Main Authors: Arvey, Aaron, Hermann, Anita, Hsia, Cheryl C., Ie, Eugene, Freund, Yoav, McGinnis, William
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2879521/
https://ncbi.nlm.nih.gov/pubmed/20164092
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkq042
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