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Minimizing off-target signals in RNA fluorescent in situ hybridization

Fluorescent in situ hybridization (FISH) techniques are becoming extremely sensitive, to the point where individual RNA or DNA molecules can be detected with small probes. At this level of sensitivity, the elimination of ‘off-target’ hybridization is of crucial importance, but typical probes used fo...

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Autors principals: Arvey, Aaron, Hermann, Anita, Hsia, Cheryl C., Ie, Eugene, Freund, Yoav, McGinnis, William
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 2010
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2879521/
https://ncbi.nlm.nih.gov/pubmed/20164092
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkq042
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