Construction of repeat-free fluorescence in situ hybridization probes

FISH probes are generally made out of BAC clones with genomic DNA containing a variable amount of repetitive DNA that will need to be removed or blocked for FISH analysis. To generate repeat free (RF) Probes without loss in genomic coverage, a random library is made from BAC clones by whole-genome a...

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Bibliografske podrobnosti
Main Authors: Swennenhuis, Joost F., Foulk, Brad, Coumans, Frank A.W., Terstappen, Leon W. M. M.
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2012
Teme:
Methods Online
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3273791/
https://ncbi.nlm.nih.gov/pubmed/22123742
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkr1123
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