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Mechanistic Approach to the Problem of Hybridization Efficiency in Fluorescent In Situ Hybridization

In fluorescent in situ hybridization (FISH), the efficiency of hybridization between the DNA probe and the rRNA has been related to the accessibility of the rRNA when ribosome content and cell permeability are not limiting. Published rRNA accessibility maps show that probe brightness is sensitive to...

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Detalhes bibliográficos
Main Authors: Yilmaz, L. Safak, Noguera, Daniel R.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC535158/
https://ncbi.nlm.nih.gov/pubmed/15574909
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.70.12.7126-7139.2004
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