Co-expression of ferrochelatase allows for complete heme incorporation into recombinant proteins produced in E. coli

Over-expression of heme binding proteins in E. coli often results in sub-optimal heme incorporation and the amount of heme-bound protein produced usually varies with the protein of interest. Complete heme incorporation is important for biochemical characterization, spectroscopy, structural studies,...

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Main Authors: Sudhamsu, Jawahar, Kabir, Mariam, Airola, Michael V., Patel, Bhumit A., Yeh, Syun-Ru, Rousseau, Dennis L., Crane, Brian R.
Formato: Artigo
Idioma:Inglês
Publicado: 2010
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Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2875318/
https://ncbi.nlm.nih.gov/pubmed/20303407
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2010.03.010
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