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Co-expression of ferrochelatase allows for complete heme incorporation into recombinant proteins produced in E. coli
Over-expression of heme binding proteins in E. coli often results in sub-optimal heme incorporation and the amount of heme-bound protein produced usually varies with the protein of interest. Complete heme incorporation is important for biochemical characterization, spectroscopy, structural studies,...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2875318/ https://ncbi.nlm.nih.gov/pubmed/20303407 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2010.03.010 |
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