Detection of tmRNA molecules on microarrays at low temperatures using helper oligonucleotides

BACKGROUND: The hybridization of synthetic Streptococcus pneumoniae tmRNA on a detection microarray is slow at 34°C resulting in low signal intensities. RESULTS: We demonstrate that adding specific DNA helper oligonucleotides (chaperones) to the hybridization buffer increases the signal strength at...

Volledige beschrijving

Bewaard in:
Bibliografische gegevens
Hoofdauteurs: Kaplinski, Lauris, Scheler, Ott, Parkel, Sven, Palta, Priit, Toome, Kadri, Kurg, Ants, Remm, Maido
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: BioMed Central 2010
Onderwerpen:
Research article
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2873282/
https://ncbi.nlm.nih.gov/pubmed/20426847
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-10-34
Tags: Voeg label toe
Geen labels, Wees de eerste die dit record labelt!

Gelijkaardige items