Detection of tmRNA molecules on microarrays at low temperatures using helper oligonucleotides

BACKGROUND: The hybridization of synthetic Streptococcus pneumoniae tmRNA on a detection microarray is slow at 34°C resulting in low signal intensities. RESULTS: We demonstrate that adding specific DNA helper oligonucleotides (chaperones) to the hybridization buffer increases the signal strength at...

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Autors principals: Kaplinski, Lauris, Scheler, Ott, Parkel, Sven, Palta, Priit, Toome, Kadri, Kurg, Ants, Remm, Maido
Format: Artigo
Idioma:Inglês
Publicat: BioMed Central 2010
Matèries:
Research article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2873282/
https://ncbi.nlm.nih.gov/pubmed/20426847
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-10-34
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