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Detection of tmRNA molecules on microarrays at low temperatures using helper oligonucleotides
BACKGROUND: The hybridization of synthetic Streptococcus pneumoniae tmRNA on a detection microarray is slow at 34°C resulting in low signal intensities. RESULTS: We demonstrate that adding specific DNA helper oligonucleotides (chaperones) to the hybridization buffer increases the signal strength at...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2873282/ https://ncbi.nlm.nih.gov/pubmed/20426847 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-10-34 |
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