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Identification of Multiple Rate-limiting Steps during the Human Mitochondrial Transcription Cycle in Vitro

We have reconstituted human mitochondrial transcription in vitro on DNA oligonucleotide templates representing the light strand and heavy strand-1 promoters using protein components (RNA polymerase and transcription factors A and B2) isolated from Escherichia coli. We show that 1 eq of each transcri...

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Detalhes bibliográficos
Main Authors: Lodeiro, Maria F., Uchida, Akira U., Arnold, Jamie J., Reynolds, Shelley L., Moustafa, Ibrahim M., Cameron, Craig E.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Biochemistry and Molecular Biology 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2871506/
https://ncbi.nlm.nih.gov/pubmed/20351113
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M109.092676
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