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Identification and purification of a single-stranded-DNA-specific exonuclease encoded by the recJ gene of Escherichia coli.

The Escherichia coli recJ gene product was overproduced using a plasmid that carries the recJ gene downstream of a strong regulatable promoter and a strong ribosome-binding site. Overexpression of recJ produced a concomitant increase in the levels of single-stranded-DNA-specific nuclease activity pr...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Lovett, S T, Kolodner, R D
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1989
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC286970/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2649886/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.86.8.2627
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