Cloning of the Escherichia coli recJ chromosomal region and identification of its encoded proteins.
A 9.6-kilobase BamHI-SalI fragment carrying recJ+ was cloned into vector pBR322. Deletion and transposon mutagenesis were used to map the recJ gene on this fragment. The maxicell protein-labeling technique was used to correlate a functional recJ gene with the presence of a polypeptide of 53,000 appa...
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| Udgivet i: | J Bacteriol |
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| Principais autores: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Microbiology (ASM)
1985
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC218986/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2984175/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.162.1.280-285.1985 |
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