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Cloning of the Escherichia coli recJ chromosomal region and identification of its encoded proteins.
A 9.6-kilobase BamHI-SalI fragment carrying recJ+ was cloned into vector pBR322. Deletion and transposon mutagenesis were used to map the recJ gene on this fragment. The maxicell protein-labeling technique was used to correlate a functional recJ gene with the presence of a polypeptide of 53,000 appa...
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| Main Authors: | , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
1985
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC218986/ https://ncbi.nlm.nih.gov/pubmed/2984175 |
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