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Hyperquenching for protein cryocrystallography

When samples having volumes characteristic of protein crystals are plunge cooled in liquid nitrogen or propane, most cooling occurs in the cold gas layer above the liquid. By removing this cold gas layer, cooling rates for small samples and modest plunge velocities are increased to 1.5 × 10(4) K s(−...

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Detalhes bibliográficos
Main Authors: Warkentin, Matthew, Berejnov, Viatcheslav, Husseini, Naji S., Thorne, Robert E.
Formato: Artigo
Idioma:Inglês
Publicado em: 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2866519/
https://ncbi.nlm.nih.gov/pubmed/20461232
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S0021889806037484
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