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Active site remodeling accompanies thioester bond formation in the SUMO E1

E1 enzymes activate ubiquitin (Ub) and ubiquitin-like (Ubl) proteins in two steps by carboxy-terminal adenylation and thioester bond formation to a conserved catalytic cysteine in the E1 Cys domain. The structural basis for these intermediates remains unknown. Here we report crystal structures for h...

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Detalhes bibliográficos
Main Authors: Olsen, Shaun K., Capili, Allan D., Lu, Xuequan, Tan, Derek S., Lima, Christopher D.
Formato: Artigo
Idioma:Inglês
Publicado em: 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2866016/
https://ncbi.nlm.nih.gov/pubmed/20164921
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nature08765
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