Analysis of the sequence-specific interactions between Cro repressor and operator DNA by systematic base substitution experiments.
We measured quantitatively the binding affinities of purified Cro repressor to the chemically synthesized wild-type and mutant OR1 operators, consisting of all three possible base-pair substitutions and of thymine to uracil substitutions at each base-pair position of the 17-base-pair operator sequen...
Uloženo v:
| Vydáno v: | Proc Natl Acad Sci U S A |
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| Hlavní autoři: | , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
National Academy of Sciences
1989
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC286485/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2911590/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.86.2.439 |
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