Generation and application of new rat monoclonal antibodies against synthetic FLAG and OLLAS tags for improved immunodetection

Previously, we prepared monoclonal antibodies (mAbs) by immunizing rats with the recombinant fusion proteins of mouse Langerin/CD207, which contained a flexible linker sequence from E. coli OmpF and a FLAG epitope. We found many of new rat mAbs were not reactive to mouse Langerin, and here we identi...

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Detalhes bibliográficos
Main Authors: Park, Sung Ho, Cheong, Cheolho, Idoyaga, Juliana, Kim, Jae Y., Choi, Jae-Hoon, Do, Yoonkyung, Lee, Haekyung, Jo, Jung Heon, Oh, Yong-Seok, Im, Wonpil, Steinman, Ralph M., Park, Chae Gyu
Formato: Artigo
Idioma:Inglês
Publicado em: 2007
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2864634/
https://ncbi.nlm.nih.gov/pubmed/18054954
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jim.2007.10.012
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