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Recursive Directional Ligation by Plasmid Reconstruction allows Rapid and Seamless Cloning of Oligomeric Genes

This paper reports a new strategy, Recursive Directional Ligation by Plasmid Reconstruction (PRe-RDL), to rapidly clone highly repetitive polypeptides of any sequence and specified length over a large range of molecular weights. In a single cycle of PRe-RDL, two halves of a parent plasmid, each cont...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: McDaniel, Jonathan R., MacKay, J. Andrew, Quiroz, Felipe García, Chilkoti, Ashutosh
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2010
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2862688/
https://ncbi.nlm.nih.gov/pubmed/20184309
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bm901387t
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