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Recursive Directional Ligation by Plasmid Reconstruction allows Rapid and Seamless Cloning of Oligomeric Genes
This paper reports a new strategy, Recursive Directional Ligation by Plasmid Reconstruction (PRe-RDL), to rapidly clone highly repetitive polypeptides of any sequence and specified length over a large range of molecular weights. In a single cycle of PRe-RDL, two halves of a parent plasmid, each cont...
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| Main Authors: | , , , |
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| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
2010
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2862688/ https://ncbi.nlm.nih.gov/pubmed/20184309 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bm901387t |
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