A streptavidin variant with slower biotin dissociation and increased mechanostability

Streptavidin binds biotin-conjugates with exceptional stability, but dissociation does occur and can be limiting in imaging, DNA amplification, and nanotechnology. We identified a mutant streptavidin, which we call traptavidin, showing ~10-fold slower biotin off-rate, increased mechanical strength,...

Full description

Saved in:
Bibliographic Details
Main Authors: Chivers, Claire E., Crozat, Estelle, Chu, Calvin, Moy, Vincent T., Sherratt, David J., Howarth, Mark
Format: Artigo
Language:Inglês
Published: 2010
Subjects:
Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2862113/
https://ncbi.nlm.nih.gov/pubmed/20383133
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1450
Tags: Add Tag
No Tags, Be the first to tag this record!

Similar Items