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FRT-seq: Amplification-free, strand-specific, transcriptome sequencing
We report an alternative approach to transcriptome sequencing for the Illumina Genome Analyzer, in which the reverse transcription reaction takes place on the flowcell. No amplification is performed during the library preparation, so PCR biases and duplicates are avoided. Since the template is poly...
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| Main Authors: | , , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2861772/ https://ncbi.nlm.nih.gov/pubmed/20081834 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1417 |
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