FRT-seq: Amplification-free, strand-specific, transcriptome sequencing

We report an alternative approach to transcriptome sequencing for the Illumina Genome Analyzer, in which the reverse transcription reaction takes place on the flowcell. No amplification is performed during the library preparation, so PCR biases and duplicates are avoided. Since the template is poly...

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Hlavní autoři: Mamanova, Lira, Andrews, Robert M., James, Keith D., Sheridan, Elizabeth M., Ellis, Peter D., Langford, Cordelia F., Ost, Tobias W.B., Collins, John E., Turner, Daniel J.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2010
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2861772/
https://ncbi.nlm.nih.gov/pubmed/20081834
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1417
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