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An improved method for large-scale preparation of negatively and positively supercoiled plasmid DNA
A rigorous understanding of the biological function of superhelical tension in cellular DNA requires the development of new tools and model systems for study. To this end, an ethidium bromide-free method has been developed to prepare large quantities of either negatively or positively supercoiled pl...
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| Main Authors: | , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2009
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2857975/ https://ncbi.nlm.nih.gov/pubmed/19594448 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/000113172 |
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