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Positively supercoiled plasmid DNA is produced by treatment of Escherichia coli with DNA gyrase inhibitors.
A procedure has been developed whereby the relative amounts of the topoisomers of E. coli plasmid can be determined for cells grown under a variety of conditions. Several applications of the procedure are presented. Addition of either novobiocin or oxolinic acid, two inhibitors of DNA gyrase, gives...
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| Publicado no: | Nucleic Acids Res |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
1983
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| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC325943/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6304617/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/11.10.2999 |
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