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Positively supercoiled plasmid DNA is produced by treatment of Escherichia coli with DNA gyrase inhibitors.

A procedure has been developed whereby the relative amounts of the topoisomers of E. coli plasmid can be determined for cells grown under a variety of conditions. Several applications of the procedure are presented. Addition of either novobiocin or oxolinic acid, two inhibitors of DNA gyrase, gives...

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Bibliografski detalji
Glavni autori: Lockshon, D, Morris, D R
Format: Artigo
Jezik:Inglês
Izdano: 1983
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC325943/
https://ncbi.nlm.nih.gov/pubmed/6304617
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