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Positively supercoiled plasmid DNA is produced by treatment of Escherichia coli with DNA gyrase inhibitors.
A procedure has been developed whereby the relative amounts of the topoisomers of E. coli plasmid can be determined for cells grown under a variety of conditions. Several applications of the procedure are presented. Addition of either novobiocin or oxolinic acid, two inhibitors of DNA gyrase, gives...
Kaydedildi:
| Asıl Yazarlar: | , |
|---|---|
| Materyal Türü: | Artigo |
| Dil: | Inglês |
| Baskı/Yayın Bilgisi: |
1983
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| Online Erişim: | https://ncbi.nlm.nih.gov/pmc/articles/PMC325943/ https://ncbi.nlm.nih.gov/pubmed/6304617 |
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