Large scale preparation of positively supercoiled DNA using the archaeal histone HMf.

A technique to prepare relatively large quantities (>/=100 microg) of highly positively supercoiled DNA is reported. This uses a recombinant archaeal histone (rHMfB) to introduce toroidal supercoils, and an inexpensive chicken blood extract to relax unrestrained superhelical tension. Preparation of...

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Bibliografiske detaljer

Udgivet i:	Nucleic Acids Res
Principais autores:	LaMarr, W A, Sandman, K M, Reeve, J N, Dedon, P C
Format:	Artigo
Sprog:	Inglês
Udgivet:	Oxford University Press 1997
Fag:	Research Article
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146619/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9092677/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.8.1660
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