Large scale preparation of positively supercoiled DNA using the archaeal histone HMf.

A technique to prepare relatively large quantities (>/=100 microg) of highly positively supercoiled DNA is reported. This uses a recombinant archaeal histone (rHMfB) to introduce toroidal supercoils, and an inexpensive chicken blood extract to relax unrestrained superhelical tension. Preparation...

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Detaylı Bibliyografya
Asıl Yazarlar: LaMarr, W A, Sandman, K M, Reeve, J N, Dedon, P C
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 1997
Konular:
Research Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC146619/
https://ncbi.nlm.nih.gov/pubmed/9092677
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