Large scale preparation of positively supercoiled DNA using the archaeal histone HMf.
A technique to prepare relatively large quantities (>/=100 microg) of highly positively supercoiled DNA is reported. This uses a recombinant archaeal histone (rHMfB) to introduce toroidal supercoils, and an inexpensive chicken blood extract to relax unrestrained superhelical tension. Preparation of...
Збережено в:
| Опубліковано в:: | Nucleic Acids Res |
|---|---|
| Автори: | , , , |
| Формат: | Artigo |
| Мова: | Inglês |
| Опубліковано: |
Oxford University Press
1997
|
| Предмети: | |
| Онлайн доступ: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146619/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9092677/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.8.1660 |
| Теги: |
Немає тегів, Будьте першим, хто поставить тег для цього запису!
|