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Analysis of the impact of a uracil DNA glycosylase attenuated in AP-DNA binding in maintenance of the genomic integrity in Escherichia coli

Uracil DNA glycosylase (Ung) initiates the uracil excision repair pathway. We have earlier characterized the Y66W and Y66H mutants of Ung and shown that they are compromised by ∼7- and ∼170-fold, respectively in their uracil excision activities. In this study, fluorescence anisotropy measurements sh...

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Détails bibliographiques
Auteurs principaux: Bharti, Sanjay Kumar, Varshney, Umesh
Format: Artigo
Langue:Inglês
Publié: Oxford University Press 2010
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2853124/
https://ncbi.nlm.nih.gov/pubmed/20056657
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkp1210
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